RNAi Facility
The Center for RNA Biology and the IGSP have established an RNAi Facility for the application of RNA interference (RNAi) technologies for mammalian functional genomics. The primary mission of the RNAi Facility is to develop and deploy RNAi technologies to support functional genomics research programs at Duke. Through investments in RNAi and complementary technologies, we have assembled state-of-the-art functional genomics infrastructure, providing researchers access to genome-wide RNAi reagents and the infrastructure necessary to conduct investigator assisted large-scale loss-of-function studies in mammalian cells. Access to RNAi reagents, expertise, and instrumentation as a shared resource provides Duke investigators with a means to manipulate the expression of individual or sets of genes experimentally, and to probe gene function on a genome-wide scale, thereby enabling and accelerating a broad range of basic and translational research programs.
- RNAi expertise: The RNAi Facility serves as the focal point of RNAi technology development and expertise for the Duke University and Medical Center research community, and will work to advance and apply gene silencing technologies to a broad range of research programs.
- RNAi reagents: The availability of RNAi libraries targeting entire genomes or large subsets provides the opportunity to conduct loss-of-function genetic screens and selections in mammalian cells. The RNAi Facility has acquired two human whole-genome RNAi collections. The Qiagen Human whole-genome siRNA library v1.0 and the Open Biosystems (Expression Arrest) shRNA based library.
- Genome-wide RNAi studies: The RNAi Facility has developed the infrastructure necessary to conduct High-throughput RNAi based screening on a genome-wide scale. In addition, we have the ability to perform High-content cell-based imaging and analysis. The Facility will work with researchers to develop, evaluate, and implement cell-based screening and/or selection studies
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